FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10771659
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA423690939.333857486576576No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGAGATCGGAA1370341.2721717239656398No Hit
TATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTT1284361.1923511503659743No Hit
TTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTT1197371.11159293104247No Hit
GGATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA976440.9064898916684978No Hit
TGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTA742170.6890025018430309No Hit
TATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT615520.5714254415220533No Hit
GGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTAC585380.5434446077433384No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAGATCGGAA458260.42543121723403976No Hit
ATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTT457740.4249484689405782No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGATCCCAGGT319320.2964445866695186No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAAGAAAAAT297680.27635483076469464No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGTTATTAATT296440.27520366175720934No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGGATCCCAGG295350.27419174706514565No Hit
ATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATT242010.22467291250122196No Hit
TTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTT191790.17805056769806768No Hit
GTATATTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT181170.16819136216621786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112280.10423649690358747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT4722450.069.6393661
CATTAAT5013250.069.5291751
ATTAATT5121350.068.190632
TATATTT4815050.068.138062
ATATTTG4938250.067.859193
GGGATCT995700.067.78316518
TTAATTT5172650.067.545463
ACCTGGG5191550.067.5077914
TGGGATC5095900.067.5028317
TGCTACC5195050.067.5000410
TACCTGG5195350.067.4755613
CTGGGAT5101050.067.45563516
TTGCTAC5199050.067.4467549
GGGATCC1079700.067.44353518
CCTGGGA5105150.067.4330615
GCTACCT5201850.067.4235511
CTACCTG5204900.067.39950612
TTTGCTA5202750.067.377268
GGGATCA2074750.067.32062518
GGATCCT234300.067.2223919