FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7687628
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGG2105622.7389722811769768No Hit
GAGCCAACTTCAAGAATAGGATCACCGACCCCATTTCTCATTAGAGCCAA1393971.813264117358436No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATCTATCAATCTATTTA427010.555450914118113No Hit
GAGCCAACTTCAAGAATAGGATCTCTTGACCAATTACGAGAATATTGGAA310780.404259935574406No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGCTATTCTTGAAGTTGGCTCA223840.2911691356553673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175940.2288612300178937No Hit
CCAGATTGCCTTTTATTGCTAAAGTAGATCCTATTCTTGAAGTTGGCTCA168240.21884513662731858No Hit
CCAGATTGCCTTTTATTGCTAGATCCTATTCTTGAAGTTGGCTCAGATCG156200.20318360878023756No Hit
CAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGC134930.17551577677796065No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGATCCTATTCTTGAAGTTGGC84890.11042417765271681No Hit
AGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGCA83080.10806974531025694No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGGATCCTATTCTTGAAGTTGG77290.10053816339708424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCG150.002225603269.9982970
CCAGATT3800300.069.202451
AGCCAAC3390650.068.062722
GCCAACT3458300.067.169523
ATAGGAT3456450.067.0275516
GAATAGG3463550.066.9877314
TAGGATC3451650.066.9052117
AGAATAG3482750.066.8540313
CCAACTT3474150.066.795574
AATAGGA3472300.066.78969615
CTTCAAG3486850.066.6908348
AGGATCG552100.066.634318
AGGATCT874900.066.6281518
CAACTTC3491350.066.490025
AAGAATA3510750.066.365712
AGGATCA1403600.066.3598918
AACTTCA3502150.066.348946
GAGCCAA3467350.066.291931
CAAGAAT3515150.066.248811
TTCAAGA3513400.066.2321249
Produced by FastQC (version 0.11.5)