FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15398199
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGT231679915.045908940389719No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTT12582488.171397187424322No Hit
TTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTT3014581.957748435385203No Hit
TTTAATGAACAAACTGCTTGATCAATAATTTTATCGGGGGAAAATACTAA1681341.091906917166092No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACGAAATGCAGTTTTTCCA1347630.8751867669719037No Hit
ATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGG864760.5615981453415428No Hit
AATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTG838750.5447065595138756No Hit
TAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTT539070.3500863964675349No Hit
TTTAATGAACAAACTGCTTGATCCATCTGGAACCATTCTCCAGATCGGAA470980.30586693937388393No Hit
GGAGAATGGTTCCAGATGGATCAAGCAGTTTGTTCATTAAAAGATCGGAA368160.23909289651341692No Hit
AGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTG363520.23607955709625522No Hit
TGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGGA332520.2159473325419421No Hit
TTTAATGAACAAACTGCTTGATCTTTCCATCTGGAACCATTCTCCAGATC308340.20024419738957785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290580.188710380999752No Hit
GGAGAATGGTTCCAGATGGAAAGATCAAGCAGTTTGTTCATTAAAAGATC220130.14295827713357906No Hit
TTAATGAACAAACTGCTTGATCAATAATTTTATCGGGGGAAAATACTAAA212920.13827591135820494No Hit
GAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTGC182420.11846840010315493No Hit
AATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTGCT182240.1183515033154202No Hit
TTTAATGAACAAACTGCTTGATCCATCTGGAACCATTCTCCAACATCGGC180560.11726046662989614No Hit
GAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGGAA174080.11305218227144617No Hit
GAGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTT173970.11298074534560829No Hit
GGAGAATGGTTCCAGATGGATCAAGCAGTTTGTTCATTAAAAGATGAAAT172460.11200011118183367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAT5660100.069.4071
TTTAATG7518000.068.986461
GAGAATG5744950.068.523392
AGAATGG5858300.067.212273
GAATGGT5946100.066.2551354
GTACGCC4300.065.9299630
CGTACCG153300.065.4563529
CGTACTC378050.065.1670429
TCGTACC994250.065.1453228
CGTACCA360300.065.11352529
CGTACAG181150.065.01491529
CGTACCT310200.065.0126329
TCGTACA1326650.064.99766528
CGTACAA500450.064.9782629
GTACCCT45850.064.9615630
CGTACAC226150.064.93893429
CGTACTA329100.064.91617629
CGTACTG307550.064.9127629
GTACTGC47400.064.904830
TGGTTCC6046700.064.8916857
Produced by FastQC (version 0.11.5)