FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4841731
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA138044428.511373308430393No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCGAGAT1027732.1226499365619445No Hit
CCATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCT990612.045983141153443No Hit
AAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGG933571.9281740352778791No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGACGTT843611.7423727175260255No Hit
AATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGG719191.4853985072694043No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA711301.4691026824910347No Hit
CAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGG611641.2632672075338345No Hit
GGTTCAAATCAGGTCCATTTTGCTCCACTCAATCATAAAAATGGACGTTT580421.1987861366110593No Hit
TTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACC460740.9516018134836487No Hit
ATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGGA376300.7772013769455594No Hit
CCATTTTTATGATTGAGTGGAGCAAAATGGACCTGATTTGAACCGAGATA361840.7473360250703726No Hit
TCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCG227070.4689851625379436No Hit
CCATTTTTATGATTGAGTGGATCTAAAAACATTTTCGAGTTTCTTATGAT182060.37602254235107235No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC169650.3503912133904176No Hit
GTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC161630.33382688959795576No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGAGATC158870.3281264489910736No Hit
CCATTTTTATGATTGAGTGGATCATAGCAAAATGGACCTGATTTGAACCG155970.32213685559978444No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCAGATC152420.3148047671380339No Hit
GGTTCAAATCAGGTCCATTTTGCTATGATCCACTCAATCATAAAAATGGA126470.26120823317115305No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGCCATT116210.24001746482817818No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCA108680.22446517578114106No Hit
GGTTCAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC100340.20723993133860596No Hit
CAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGGAAA89860.18559478004870572No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT83470.1723970208175547TruSeq Adapter, Index 19 (97% over 40bp)
CATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTC77180.15940579928955162No Hit
GGGGCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA75790.15653492521579576No Hit
CCATTTTTATGATTGAGTGGATCAGCAAAATGGACCTGATTTGAACCGAG73880.1525900550856708No Hit
ATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTCA71740.14817014823830568No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCACA69290.14310997451118204No Hit
GGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC68000.14044563814057412No Hit
GGTTCAAATCAGGTCCATTTTGCTCCACTCAATCATAAAAATGGAGATCG64910.1340636231133039No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATAGA51860.10711045285250254No Hit
CCATTTTTATGATTGAGTGGAGCAAAATGGACCTGATTTGAACCAGATCG49930.10312427518174802No Hit
CCATTTTTATGATTGAGTGGATCATAGCAAAATGGACCTGATTTGAACCA49730.1027111997754522No Hit
CCATTTTTATGATTGAGTGGATCAAGCAAAATGGACCTGATTTGAACCGA48990.10118282077215773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTA150.002225417669.9993630
GGTTCAA1817100.068.8378141
GGGTCAA101000.067.75251
GTTCAAA1838350.067.5821462
TTCAAAT1901000.065.2333763
TCAAATC2002000.065.197634
GGATGCA142200.063.9452319
TGCATCC153700.063.89727821
CATCCAC155150.063.14219723
GCATCCA155050.063.115222
TGGATGC144400.063.0194718
CAAATCA2079100.062.9983445
CATTTTT2099250.062.535742
GTGGATG145800.062.53436717
TTGCATC158750.062.04101620
GATGCAA147600.061.60576220
GCTCCAC100400.061.56381222
CTCCACT101200.061.42299323
TTGCTCC101250.061.21982620
AAATCAG2182050.060.0581366
Produced by FastQC (version 0.11.5)