FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14402460
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG425020.295102364457183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299300.20781172105320897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG7619500.069.795381
GAGAACA6303150.069.353631
ACGAGGT7698500.069.18412
CGAGGTA7731400.069.016183
AGAACAG6368450.068.975642
GAGGTAA7764600.068.7368554
GGTAAAC7769400.068.728316
AGGTAAA7784200.068.5843355
GTAAACT7791050.068.53737
GAACAGT6430650.068.455263
TTTGATC7807100.068.4200817
TAAACTA7812300.068.395658
GTACGCT12850.068.3661330
AACAGTG6449350.068.283914
CTGTACT2769950.068.2741128
AACTAAT7824300.068.25973510
ACTGTAC6415500.068.25490627
CTGTACC1855450.068.2100128
CTGTACG239700.068.2041428
TGTACTT1048250.068.1940329
Produced by FastQC (version 0.11.5)