FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10578719
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG1847331.7462700351526492No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACGGGACCTTATTTTTCAA506370.4786685420039988No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTTGCTATTATAAAATAA334050.3157754733819851No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACGAACATCAAACGTTTCA210670.19914509497794583No Hit
AACGAGGTAAACTAATTTTGATCGCAATCGACGTCGTCAAGCTGGCAAAA199520.18860506645464353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168400.1591875159931935No Hit
AACGAGGTAAACTAATTTTGATCGAGTACAGTTGAAATATATTTCGAACA136740.12925950675124276No Hit
AACGAGGTAAACTAATTTTGATCAGTACAGTTGAAATATATTTCGAACAC124360.11755676656124432No Hit
AACGAGGTAAACTAATTTTGATCTTGAGAAAATGAAATATTGAATTTCAA119610.11306661988091375No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTCGATCAAAATTAGTTT115250.10894513787538926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG5723750.069.792341
GAGAACA4410450.069.355091
ACGAGGT5794350.069.100132
AGAACAG4456850.068.873042
CGAGGTA5840100.068.7131043
GAGGTAA5865150.068.448284
GGTAAAC5875150.068.366436
AGGTAAA5879200.068.29075
GAACAGT4507150.068.27573
GTAAACT5882750.068.273297
TAAACTA5897750.068.166078
GTACTTG181050.068.1245930
AACTAAT5907750.068.0158310
CTGTACT1877850.067.9800228
ACTAATT5912850.067.95479611
GTACACG23700.067.9322630
AACAGTG4534650.067.8963854
GTACGTG14900.067.88566630
TGTACTA376750.067.8448129
GTGTTCG4550350.067.821348
Produced by FastQC (version 0.11.5)