FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13373997
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATTT215958816.14766326028038No Hit
ATGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGTCC214934116.071044430472057No Hit
ATGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGTC178635213.356904446740941No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATTTGC11852348.862227201037955No Hit
CATGTAAAGAGTTTTTTAGGATTAGGATTACTCGGGTCAAAACACATTTG11364858.497721361833714No Hit
CATGTAAAGAGTTTTTTAGGATCAGGATTACTCGGGTCAAAACACATTTG8708816.511748133336654No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGTCCG8688056.496225473955168No Hit
ATGTGTTTTGACCCGAGTAATCCTGATCCTAAAAAACTCTTTACATGTCC8247946.1671465905069365No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC2168951.6217664771421738No Hit
CATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATAG837820.6264544548649144No Hit
ATGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGAG681860.5098401024016979No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTC631530.47220737375670113No Hit
ATGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGAGA544050.40679686110292984No Hit
CATGTAAAGAGTTTTTTAGGATTAGGATTACTCGGGTCAAAACACATAGA390780.2921938744266206No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATAGAT352610.2636534164019926No Hit
CATGTAAAGAGTTTTTTAGGATCAGGATTACTCGGGTCAAAACACATAGA307280.22975928587392383No Hit
ATGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCCGCAAGG298500.2231943075805984No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGAGAT281700.21063261790772048No Hit
TGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGTCCG274470.20522660503064266No Hit
ATGTGTTTTGACCCGAGTAATCCTGATCCTAAAAAACTCTTTACATGAGA260980.19513986731117108No Hit
TGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGTCC253370.18944972097720672No Hit
ATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATTTG233310.17445046533209183No Hit
TGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATTTGC217670.16275613042234122No Hit
GTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGTCCGC208570.15595188184953235No Hit
GTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGTCCG203850.15242264522715238No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC183030.13685512266826438No Hit
TGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTA163630.12234936197458397No Hit
TGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATTTGCAA161410.12068942441066796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158670.11864067264259143No Hit
TGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGTCCGC150010.11216542070407223No Hit
ATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATTTGCA144460.10801557679428221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTC150.00222464470.006181
CGTACCT207.9318954E-569.9996429
GTACCAC252.8427967E-669.9993830
TAGTACC950.069.9993828
ACGGACG150.002225501769.99937440
TCGTACG150.002225501769.99937428
TACCCGC150.002225567869.99885667
CATGTAA6092650.069.917711
ATGTGTT6405850.069.879961
ATGTAAA6160550.069.216352
TGTGTTT6542400.068.925112
GGATTAG1244800.068.6318919
TGTAAAG6228250.068.479513
GATTAGG1248150.068.4196520
ATTAGGA1249750.068.31753521
GTGTTTT6620850.068.1081853
GTAAAGA6279100.068.0397644
TAAAGAG6299200.067.868335
AAAGAGT6311250.067.740116
CTAGGAT2393000.067.69701423
Produced by FastQC (version 0.11.5)