FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10755483
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG412910438.39068873057584No Hit
TCATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAA141948613.197789443765565No Hit
GGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGG2835372.636208899219124No Hit
CATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAAT974300.905863548852246No Hit
GGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG414730.38559867557784244No Hit
GCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGGT183050.17019226379698615No Hit
TGGCGTTAAATTCTAGGTGGAAGATCATTGTATTTGGATGCATGAAGATC172960.16081100216512823No Hit
TCATGCATCCAAATACAATGATCGGGCTTTATTTAAAAAAACAAAATGGG169930.15799383440055645No Hit
TGGCGTTAAATTCTAGGTGGAAGAGATCATTGTATTTGGATGCATGAAGA151460.1408211978950643No Hit
ATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAATC141110.13119819909528935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCA5419500.069.531451
TGGCGTT4356850.069.521091
CATGCAT5802950.065.4872742
GGCGTTA4715000.065.058422
AGGCCGT4614300.065.0212639
GGCCGTT4613600.065.01292440
AAGGCCG4617650.065.0059338
ATGCATC5846950.064.984283
CGACTTA4579700.064.9813764
GAAGGCC4618600.064.977437
GGTCGAC4580000.064.9732861
GACTTAT4587050.064.9489765
TGAAGGC4624800.064.9485736
GGTCTCG4611000.064.94134550
GTCTCGT4609100.064.934751
CCGTTTA4619800.064.9340142
TGGTCGA4585800.064.92850560
GTCGACT4582850.064.9252562
TGGTCTC4614450.064.9064449
GCCGTTT4622400.064.90581541