FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11137621
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGG10287599.236793027882705No Hit
GAGCCAACTTCAAGAATAGGATCACCGACCCCATTTCTCATTAGAGCCAA4860914.364406007351121No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATCTATCAATCTATTTA1289641.15791334612661No Hit
GAGCCAACTTCAAGAATAGGATCTCTTGACCAATTACGAGAATATTGGAA707950.6356384366104755No Hit
CAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGC339240.30458928347445113No Hit
AGCCAACTTCAAGAATAGGATCACCGACCCCATTTCTCATTAGAGCCAAA144930.13012653240759403No Hit
AGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGCA132890.11931632437483732No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGTACAAGAAAACGCTGTGAGC119170.10699771522123082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGATT6251950.069.6068651
AGCCAAC4379450.068.3270652
GTACAGC34750.067.6823730
GCCAACT4457100.067.638983
GTACGCT15950.067.5851330
GAATAGG4469350.067.4985214
ATAGGAT4460850.067.4907916
TAGGATC4464700.067.4040717
GTACGCA21400.067.38209530
AGAATAG4489800.067.3407613
CAGATTG6474400.067.299472
AATAGGA4479600.067.2870415
GTACAGA75850.067.18416630
CCAACTT4486200.067.165124
GTACCGT65950.067.1331230
AGTACAG234750.067.0626729
GTACGGC8200.067.0111230
AGTACGC52500.066.9998329
GTACTAA114350.066.9993730
GTACACG43100.066.9942930