FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12083039
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGT348298628.825413871460647No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTT167819613.888856934087526No Hit
TTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTT2110111.7463404694795737No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACGAAATGCAGTTTTTCCA946730.7835197751161773No Hit
ATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGG801100.6629954599997567No Hit
AATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTG777790.6437039556025599No Hit
TTTAATGAACAAACTGCTTGATCAATAATTTTATCGGGGGAAAATACTAA711330.5887012365018436No Hit
TAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTT655980.5428932241301216No Hit
TGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGGA458960.3798382178523135No Hit
AGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTG428510.3546376040001195No Hit
GGAGAATGGTTCCAGATGGATCAAGCAGTTTGTTCATTAAAAGATCGGAA293760.24311764614845652No Hit
GAGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTT247770.20505602936479803No Hit
TTTAATGAACAAACTGCTTGATCCATCTGGAACCATTCTCCAACATCGGC227160.18799906215646575No Hit
GGAGAATGGTTCCAGATGGAAAGATCAAGCAGTTTGTTCATTAAAAGATC217910.18034370326868926No Hit
GAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTGC207950.17210074386087804No Hit
AATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTGCT172010.14235657105799293No Hit
GAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGGAA166430.13773852753433966No Hit
TTTAATGAACAAACTGCTTGATCCATCTGGAACCATTCTCCAGATCGGAA158940.13153975585115632No Hit
TTTAATGAACAAACTGCTTGATCCAAATGCTCAAAAGTTCTCTCACAAAA137460.11376277110418995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAT4164600.069.5781
TTTAATG6588300.069.477181
GAGAATG4240900.068.42622
AGAATGG4319150.067.21033
CGTACGG10750.066.7436529
TGTACGT2000.066.50001525
GAATGGT4370900.066.4714054
CGTACAT158050.065.8583729
TTAATGA6995350.065.778032
CGTACTC243500.065.75922429
CGTACCG79300.065.67412629
CGTACTG160150.065.6504229
GTACAAC41700.065.63496430
TCGTACA522600.065.6063328
CGTACAA201600.065.5897529
CGTACCA231900.065.5773129
ATGGTTC4426700.065.5218056
TGGTTCC4423900.065.511037
TCGTACC650600.065.4754628
CGTACTA186950.065.4688529
Produced by FastQC (version 0.11.5)