FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7897980
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA300973238.10761739077587No Hit
CATTAATTTTGCTACCTGGGATCTTACAAAAATCTGAATTTTTTTTTTCA2193822.7776975884973116No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGAGATCGGAA1576221.9957254893023282No Hit
TTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTT973151.2321504992415782No Hit
TATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTT946801.1987875380793571No Hit
GGATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA905661.146698269684147No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAGATCGGAA549740.6960513954200948No Hit
GGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTAC504330.6385556813261113No Hit
TGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTA470640.5958992046067475No Hit
TATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT447150.5661574225308244No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGTTATTAATT385750.4884160253634474No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAAGAAAAAT380030.48117366719085136No Hit
ATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTT306630.38823851161942674No Hit
TTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTT294760.37320935226475627No Hit
GTATATTTGGCTCGAAAACTGCAGTTCTGCCGAATTTGCCGGTTTGCCGA203380.2575088820179337No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGATCCCAGGT181150.22936244457443547No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGGATCCCAGG177660.22494359317192497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC177450.22467770239985416TruSeq Adapter, Index 11 (100% over 50bp)
GCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTACC164740.20858497995690037No Hit
CATTAATTTTGCTACCTGGGATCCGGAGGAGGCGGCGGGCGAGAAGACGT163190.2066224528297109No Hit
ATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATT158620.20083616317083608No Hit
CATTAATTTTGCTACCTGGGATCAAGTTACTATTGCTCGTGACGATGTCA146120.18500933149995316No Hit
GTATATTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT129970.1645610649811724No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATCTGTTCGAGGAGCC121940.15439390831579722No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACTCATTAATT85990.10887594043033788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81250.10287440586073907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACA207.9319776E-569.9991464
GTATATT3614950.069.620591
CATTAAT3352350.069.323261
TATATTT3680850.068.245592
ATATTTG3798650.068.035583
ATTAATT3452300.067.5058752
GGGATCT897900.067.2060218
GATCTTA277250.066.8827520
ACCTGGG3501350.066.7772714
GGATCTT417200.066.7630419
TACCTGG3504450.066.7591513
GCTACCT3506050.066.7403611
TGCTACC3506550.066.7358310
TTGCTAC3508050.066.706299
TGGGATC3383600.066.6690617
TTTGCTA3509300.066.662588
CCTGGGA3387400.066.65645615
GGGATCA1242450.066.6486218
CTACCTG3511700.066.6478112
CTGGGAT3386800.066.63251516
Produced by FastQC (version 0.11.5)