FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8111609
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG1590281.960498835681059No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACGGGACCTTATTTTTCAA425640.5247294340740536No Hit
AACGAGGTAAACTAATTTTGATCAGAAGGGTACAGTTGAAATATATTTCG235110.2898438521876486No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTTGCTATTATAAAATAA228640.2818676294678405No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACCCTTCTGATCAAAATTA179070.2207576819839319No Hit
AACGAGGTAAACTAATTTTGATCCAACACCACAAAACAATGGAGATAACA165440.20395460382767466No Hit
AACGAGGTAAACTAATTTTGATCAACGTGGCAAATAGAACTTACATGATA141440.17436737890102938No Hit
AACGAGGTAAACTAATTTTGATCTGCCACAAAACAAAATCACAAAATTAA138330.1705333676709516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128200.15804509314983006No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACCATGTCTAAAACAATTA119680.147541628300871No Hit
AACGAGGTAAACTAATTTTGATCCAACAAGCACAATAACTACAAGTACAG117400.14473084193283972No Hit
AACGAGGTAAACTAATTTTGATCGTTTCTTACGATTAGTACAGTTGAAAT115890.1428693123645383No Hit
AACGAGGTAAACTAATTTTGATCACGGGTTACATGTAACTAGTTGACCTT113330.13971334170569613No Hit
AACGAGGTAAACTAATTTTGATCAATCGAACGATGGTACAGTTGAAATAT112790.13904762914484659No Hit
AACGAGGTAAACTAATTTTGATCATGTGTATGATAAACATTCTCAAGGAG104300.12858114832704584No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTAATCGTAAGAAACGAT101490.12511697740855113No Hit
AACGAGGTAAACTAATTTTGATCTTCATCATCACCCATTAAAACAAGACA96440.11889133216356954No Hit
AACGAGGTAAACTAATTTTGATCGCTCTGTCAAAGCTGATAAGGCTGATA90200.11119865368264174No Hit
AACGAGGTAAACTAATTTTGATCACATGGGTACAGTTGAAATATATTTCG88640.10927548406240982No Hit
AACGAGGTAAACTAATTTTGATCTGTTAAAGCGAGGTACAGTTGAAATAT88490.10909056390661828No Hit
AACGAGGTAAACTAATTTTGATCGGGAGAGAAAAAAGAAATGGATGGTAC85990.10600856131009273No Hit
AACGAGGTAAACTAATTTTGATCTGAAGCTTGTGAAAATTGGTCAAATGT84980.10476343226109641No Hit
AACGAGGTAAACTAATTTTGATCAAACCAAAATGGGACACTCTGACACCA83910.10344433514978349No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTGGCCAATAAGATATCC83740.10323475897321974No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACCACTACATTTGCACAAT82920.10222386212155937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA353.7180143E-969.9991856
GTACGCC1250.069.99917630
AACGAGG4345050.069.874771
GAGAACA3345900.069.203691
ACGAGGT4420150.068.890272
AGAACAG3391050.068.579412
CGAGGTA4469350.068.3723143
GGTAAAC4484200.068.2095956
GAGGTAA4482700.068.17654
AGGTAAA4493000.068.044845
GTAAACT4496950.068.015437
TAAACTA4507600.067.9020848
TTTGATC4499100.067.83217
AACTAAT4514800.067.7861710
GAACAGT3446500.067.7319643
ACTAATT4532350.067.5391411
TTGATCT1420650.067.4682318
TTGATCG654850.067.3979918
CTAATTT4552150.067.2695612
GCGTACA2450.067.1424967
Produced by FastQC (version 0.11.5)