FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12292209
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGG10388318.451133559476576No Hit
GAGCCAACTTCAAGAATAGGATCACCGACCCCATTTCTCATTAGAGCCAA5277474.29334548411925No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGTACATCTATCAATCTATTTA2573062.093244590943743No Hit
GAGCCAACTTCAAGAATAGGATCTCTTGACCAATTACGAGAATATTGGAA1560771.269722960291352No Hit
CAGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGC424840.34561729303496225No Hit
CCAGATTGCCTTTTATTGCTAAACTACTAGTACAAGAAAACGCTGTGAGC335510.27294524523623054No Hit
GAGCCAACTTCAAGAATAGGATCATGTTTCGAGGTGGATTGCAGTAGAGT172650.14045481979683228No Hit
AGCCAACTTCAAGAATAGGATCACCGACCCCATTTCTCATTAGAGCCAAA154770.12590902090909778No Hit
AGATTGCCTTTTATTGCTAAACTACTAGTACATAGGCAAACTGCCGGGCA126240.1026991975160852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGATT6534050.069.6290361
AGCCAAC5152600.068.514352
GCCAACT5230550.067.9141853
GAATAGG5250450.067.797614
TAGGATC5240300.067.7796917
ATAGGAT5246300.067.7675616
AGAATAG5264950.067.66513
AATAGGA5262750.067.6074915
CCAACTT5254350.067.589244
CAACTTC5279050.067.3047565
AGGATCT1291950.067.24261518
AAGAATA5302700.067.22790512
AACTTCA5289800.067.216946
CAAGAAT5313050.067.0590511
CTTCAAG5306950.067.046938
ACTTCAA5306700.067.02367
AGGATCG757050.066.7688418
TCAAGAA5338700.066.7230910
CAGATTG6825350.066.7185442
AGGATCA2309300.066.713118