FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13126770
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGT192656514.676611230333128No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTT8226846.267223391588335No Hit
TTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTT1353131.030817177416836No Hit
GGAGAATGGTTCCAGATGGAAAGGTGTTCGTACGAAATGCAGTTTTTCCA1067180.812979887664673No Hit
TTTAATGAACAAACTGCTTGATCAATAATTTTATCGGGGGAAAATACTAA678540.5169131477126513No Hit
AATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTG421630.32119858883792435No Hit
ATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGG409430.3119046041029134No Hit
TTTAATGAACAAACTGCTTGATCCAAATGCTCAAAAGTTCTCTCACAAAA334180.2545790015365547No Hit
TAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTT328610.250335764243603No Hit
TGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGTTTGGA198320.15108057808585051No Hit
AGAATGGTTCCAGATGGAAAGGTGTTCGTACTTGTTTTGTTTGGGGTTTG180390.13742146773349423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAATG6791300.069.4668051
GGAGAAT5012250.069.462571
GAGAATG5089950.068.51922
AGAATGG5151550.067.727223
GAATGGT5193900.067.224854
CGTACAA461350.066.9874229
CGTACTG306950.066.9319629
TCGTACA1217150.066.90250428
CGTACAT372650.066.8998229
CGTACCT328550.066.88859629
CGTACTA297650.066.84788529
TCGTACC1041350.066.80652628
CGTACCC202550.066.802529
CGTACTC389100.066.7160229
TTCGTAC5101100.066.6752127
TGGTTCC5226150.066.656847
ATGGTTC5230750.066.653346
GGTTCCA5229100.066.617388
CGTACAG168400.066.61146529
CGTACCA358750.066.5846129
Produced by FastQC (version 0.11.5)