FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10199308
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA389013638.141175852322526No Hit
CATTAATTTTGCTACCTGGGATCTTACAAAAATCTGAATTTTTTTTTTCA6109405.9900142244944465No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGAGATCGGAA1965001.9266012949113804No Hit
GGATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA1146741.1243311801153568No Hit
TATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTT784290.7689639336315758No Hit
TTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTT685010.671623996451524No Hit
TATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT433460.4249896169426396No Hit
TGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTA388840.3812415508973746No Hit
ATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTT338610.33199311168953816No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAGATCGGAA301720.29582399119626546No Hit
ATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATT222650.21829912382291036No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGATCCCAGGT197580.1937190248593336No Hit
GGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTAC197290.1934346918437996No Hit
TTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTT158720.15561840077777825No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGGATCCCAGG144160.1413429224806232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132030.12944995876190815No Hit
CATTAATTTTGCTACCTGGGATCTTACAAAAATCTGAATTTTTTTTTTAA118560.11624318041969121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT4361150.069.689081
CATTAAT5009850.069.611151
ATTAATT5071200.068.910232
GGGATCT1641450.068.6929618
ACCTGGG5113100.068.5878814
TACCTGG5115200.068.5655413
TGCTACC5116450.068.5614110
GATCTTA715850.068.55247520
TGGGATC5071650.068.5356117
GCTACCT5119200.068.527311
TTGCTAC5120150.068.518029
TATATTT4436250.068.492112
CCTGGGA5078800.068.48615
CTGGGAT5077100.068.4830716
CTACCTG5123750.068.4804912
TTTGCTA5124050.068.4624568
TTAATTT5107950.068.44873
GGGATCA1660500.068.3454118
GGGATCC953550.068.24554418
ATATTTG4587100.068.217463
Produced by FastQC (version 0.11.5)