FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19154809
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC719524637.5636530753191No Hit
GAATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT366730119.145588974549423No Hit
TTTAGGGTCTCCGCATAACACCCTAAAGATCTCAAGGTCTAATAAAATTC4171232.177641134401288No Hit
GAATTTTATTAGACCTTGAGATCTTTAGGGTGTTATGCGGAGACCCTAAA2833271.4791429139282986No Hit
GGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACCT2713111.4164119308106908No Hit
GGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACC2028421.0589612248287101No Hit
GTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACCTG1315130.6865795425054878No Hit
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCCCC1133400.5917051952854241No Hit
TTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCT933710.4874546125727487No Hit
GTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC847660.4425311680215658No Hit
AGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTAC719160.3754461869079457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC431910.2254838458582385TruSeq Adapter, Index 6 (100% over 50bp)
GAATTTTATTAGCCCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT362080.1890282487285569No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313550.16369257453833136No Hit
TAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTA312130.1629512463423676No Hit
TTTAGGGTCTCCGCATAACCCCCAATTTCCATTTCCAAATTACCCTCACC303370.1583779822602251No Hit
AATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTTG265370.13853962208654755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTT8936150.069.256271
TTAGACC8969400.068.967449
ATTAGAC8985050.068.95128
TAGACCT8967950.068.9506710
AGACCTT8966900.068.935911
GAGATCT5755800.068.935418
GACCTTG8964800.068.92609412
TGAGATC9045550.068.8827117
CCTTGAG9048150.068.86056514
TCCGGTA3938800.068.8201570
GTTCCGG3965800.068.8108968
GATCTTC4145550.068.808120
TATTAGA9010600.068.8045967
AGATCTT4784000.068.8042219
AATTTTA9012200.068.8042142
CTTATCT3974300.068.7887826
CTTGAGA9046550.068.7822115
GTGCAAC3966850.068.7783833
TTATTAG9086500.068.7737356
TATCTGT3980900.068.7600128
Produced by FastQC (version 0.11.5)