FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143866
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA218915219.644457318492524No Hit
CCATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCT191141917.152207321947337No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCAGATCGGA8629667.743865548993501No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGAGATCGGA7388856.630418922840602No Hit
CCATTTTTATGATTGAGTGGATCAAGCAAAATGGACCTGATTTGAACCAG4700444.217961701980264No Hit
GGTTCAAATCAGGTCCATTTTGCTTGATCCACTCAATCATAAAAATGGAG3857913.461913486755853No Hit
CCATTTTTATGATTGAGTGGATCAGCAAAATGGACCTGATTTGAACCAGA2500902.2441942500026473No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCAGATC2498382.241932916278785No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGAGATC2258672.0268280325696666No Hit
GGTTCAAATCAGGTCCATTTTGCTGATCCACTCAATCATAAAAATGGAGA2031621.823083658758998No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGCCATTTTT1148881.0309528129645493No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA753860.6764797782026453No Hit
CCATTTTTATGATTGAGTGGATCGCAAAATGGACCTGATTTGAACCAGAT614240.5511911216448583No Hit
CCATTTTTATGATTGAGTGGATGGCAAAATGGACCTGATTTGAACCAGAT508310.45613434332394165No Hit
GGTTCAAATCAGGTCCATTTTGCTTGATCCACTCAATCATAAAAATGGCC507840.4557125866373483No Hit
GGTTCAAATCAGGTCCATTTTGCGATCCACTCAATCATAAAAATGGAGAT497070.44604807703179483No Hit
GGTTCAAATCAGGTCCATTTTGCCATCCACTCAATCATAAAAATGGAGAT475920.4270690261350953No Hit
CATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTC472200.4237308668284418No Hit
CCATTTTTATGATTGAGTGGATCATAGCAAAATGGACCTGATTTGAACCA440130.3949527031283398No Hit
AAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGG435670.39095050137896487No Hit
AATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGG366800.32914968647325804No Hit
GGTTCAAATCAGGTCCATTTTGCTCCACTCAATCATAAAAATGGAGATCG353200.31694566320162143No Hit
GGTTCAAATCAGGTCCATTTTGCTATGATCCACTCAATCATAAAAATGGA338090.3033866344049722No Hit
CAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGG315980.28354612304203947No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGCCATT313660.28146426024864263No Hit
TTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACC310470.27860169890772196No Hit
ATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTCA293540.263409484643839No Hit
GGTTCAAATCAGGTCCATTTTGCTGATCCACTCAATCATAAAAATGGCCA287120.25764846777590467No Hit
CCATTTTTATGATTGAGTGGAGCAAAATGGACCTGATTTGAACCAGATCG276330.24796601107730476No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGAGGTTCAA228160.2047404374747507No Hit
CCATTTTTATGATTGAGTGGATCAATTTTCGCTTTTCATTGGAAAGAAAA221440.1987102142111185No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC217750.1953989755440347No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGGATCGGAA216190.19399910228640582No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCTCCATTTT216100.19391834036769645No Hit
ATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGGA206210.18504350285619012No Hit
CCATTTTTATGATTGAGTGGATCTTATCATGCAAAATGGACCTGATTTGA186120.16701564789095633No Hit
GGTTCAAATCAGGTCCATTTTGCGCGTTTGGACCAAGACCCATTCCAGGC179390.16097645108080086No Hit
GGTTCAAATCAGGTCCATTTTGCATGATAAGATCCACTCAATCATAAAAA174350.15645378363307672No Hit
CCATTTTTATGATTGAGTGGATCAGGGCCTGCAGGAATGGGACAACCAAT170100.15264002636069027No Hit
GTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC160550.14407028943097486No Hit
GGTTCAAATCAGGTCCATTTTGCTGGTTGAGCGTCTTGTCGGGGGAGCTG144640.12979337691246465No Hit
CCATTTTTATGATTGAGTGGATCTAAAAACATTTTCGAGTTTCTTATGAT139740.12539633911606618No Hit
TCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCG124820.11200780770335897No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCTGGTTCAA121230.10878630450150781No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCAGGTTCAA116430.10447900217034195No Hit
CCATTTTTATGATTGAGTGGATCAAGCAAAATGGACCTGATTTGAACCTC115080.10326757338970156No Hit
CCATTTTTATGATTGAGTGGATCAACTAAAAGTCGAAGTTGAAGATAGTA114540.10278300187744539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCAA112000.067.755291
GGTTCAA5016600.067.267541
GTTCAAA5057750.066.735532
TTCAAAT5134150.065.831083
TCAAATC5240000.065.8171164
CAACGAG2153200.065.2658239
GCTCTCA2163950.065.2618434
GAGTTCC2151000.065.2414543
ACGAGTT2157850.065.2338541
GTTCCTC2152950.065.21162445
AGTTCCT2151750.065.2073244
TGCTCTC2170150.065.2060233
CGAGTTC2152900.065.20338442
GTGCTCT2174200.065.18114532
CCTCAAC2138050.065.166848
TCAACGA2156050.065.1649338
GGTGCTC2170650.065.1619831
CAAATCA5304950.065.157835
TCCTCAA2141350.065.15626547
CTCTCAA2162000.065.1539535
Produced by FastQC (version 0.11.5)