FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5174650
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC176208834.0523127168021No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTC125529324.25851023740736No Hit
ATGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGTC1013081.9577749219753995No Hit
CATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATTT952271.8402597277110528No Hit
ATGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGAG938641.8139197820142425No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC874831.6906070941996076No Hit
CATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATAG813821.5727054003652419No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC669611.2940198854028775No Hit
TGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTA254980.4927483018175143No Hit
ATGTGTTTTGACCCGAGTAATCCTGATCCTAAAAAACTCTTTACATGTCC242190.46803165431478455No Hit
TGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG230970.44634902843670593No Hit
CATGTAAAGAGTTTTTTAGGATCAGGATTACTCGGGTCAAAACACATTTG228410.4414018339404598No Hit
ATGTGTTTTGACCCGAGTAATCCTGATCCTAAAAAACTCTTTACATGAGA218010.4213038562994599No Hit
ATGTGTTTTGCCCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC214540.4145980887596263No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCCCCTGTCAATCC188790.3648362691196506No Hit
GTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGT182530.3527388325780487No Hit
CATGTAAAGAGTTTTTTAGGATCACTGGTTTGTCGCTAGAAACTTTCTGT178710.3453566907906815No Hit
CATGTAAAGAGTTTTTTAGGATCAGGATTACTCGGGTCAAAACACATAGA176100.34031287140193056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC165410.31965446938440284TruSeq Adapter, Index 4 (100% over 50bp)
GTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAG140470.271457973003005No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGTCCG101010.19520160783821128No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATTTGC98840.19100808750350265No Hit
GGGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC98780.19089213763249688No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC96850.18716241678181134No Hit
ATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTCT94080.18180939773704502No Hit
TGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTCTG91510.17684287826229794No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACACCGACCACCTGTCAATCC89980.17388615655165085No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGAGAT88100.1702530605934701No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATAGAT78060.15085078217850484No Hit
TTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGG65050.12570898514875403No Hit
ATGTGTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG64870.12536113553573672No Hit
CATGTAAAGAGTTTTTTAGGATCTGTGAAATCGAAACGTTTAACTTTTGC64560.12476206120220691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGC650.070.0004943
GTACGTA252.8422837E-670.0004943
GTACCGG700.070.0004843
CGTACGA1100.069.999850
CCGTACG1100.069.9991349
CATGTAA2295350.069.812581
ATGTGTT2313250.069.801951
ATGTAAA2323050.069.109382
TGTGTTT2483150.069.035712
TGTAAAG2348150.068.5309753
GTAAAGA2371850.068.272654
GGTACGA1950.068.205642
GTGTTTT2526600.068.177533
TAAAGAG2383900.067.946335
TAGATCC209300.067.9088624
TAAGGTA1407800.067.8890839
ATAAGGT1410150.067.8826638
CACTTTA1407950.067.8644329
AATAAGG1413700.067.8576137
TATCACT1406000.067.8515226
Produced by FastQC (version 0.11.5)