FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11598166
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG517348644.606069614799445No Hit
TCATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAA263656422.732594101515705No Hit
GGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGG1711451.4756212318395856No Hit
CATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAAT867800.7482217447137762No Hit
TCATGCATCCAAATACAATGATCGGGCTTTATTTAAAAAAACAAAATGGG756160.6519651469033984No Hit
GGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG734710.6334708435799247No Hit
TCATGCATCCAAATACAATGATCGGACATGTCTCTGCGAGTATCAAATAC519050.4475276522167384No Hit
TCATGCATCCAAATACAATGATCATTCGATGGTTGGCATCCTAAAAATTT268970.23190735500767967No Hit
ATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAATC193690.16700054129247674No Hit
GCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGGT158220.1364181198993013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133850.11540617714904235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTT5345900.069.91031
TCATGCA5456350.069.837241
GGCGTTA5618650.067.736992
CATGCAT5691050.067.638492
GCGTTAA5641250.067.515263
CTAGGTG5651400.067.4544113
CGTTAAA5647850.067.443184
TAGGTGG5653050.067.43471514
TCTAGGT5656050.067.4221712
AGGCCGT5602850.067.4167739
TTCTAGG5656750.067.4140511
AAGGCCG5608700.067.4119738
GTCGTGC5616700.067.4072323
AGTCGTG5620250.067.4063722
GGCCGTT5602450.067.4047240
GAAGGCC5607800.067.4046937
CGTGCAT5613550.067.4023725
GGAAGTC5637850.067.40172619
GGTCGAC5558900.067.3976661
ATTCTAG5657400.067.39764410