FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798837
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA149848339.44583565970322No Hit
CCATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCT62886816.554224358665557No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA684791.8026306472217681No Hit
CCATTTTTATGATTGAGTGGATCTAAAAACATTTTCGAGTTTCTTATGAT233660.615082984608184No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC171530.45153292968347947No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGAGATC112750.296801363154039No Hit
TTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACC94880.24976065043064496No Hit
GTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC83900.22085706757094342No Hit
CAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGG83720.2203832383437352No Hit
CATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTC82240.21648730914224537No Hit
AAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGG70150.18466177938142647No Hit
GGGGCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA67690.17818611327624745No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCACA67330.177238454821831No Hit
TCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCG64780.17052587410304787No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCAGATC57350.1509672565577307No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC55060.14493909583380388TruSeq Adapter, Index 2 (100% over 50bp)
AATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGG43020.11324518530276503No Hit
CCATTTTTATGATTGAGTGGATCAATTTTCGCTTTTCATTGGAAAGAAAA40910.10769085380604644No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC40320.1061377468946417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG353.7161954E-970.0017128
GTACGCT150.002225433269.9989544
GTACGAA207.931556E-569.9989563
GGTTCAA1647800.069.6415251
GTTCAAA1663400.068.928872
GGGTCAA87450.068.561761
TCAAATC1762600.068.333864
GAGTAGG679150.068.31647526
TAGGTGC678650.068.3082829
TAGCGGG673750.068.3002763
GTAGCGG673850.068.2849462
AGCGGGT667950.068.269864
GCGGGTT667500.068.2529165
AGTAGCG674000.068.2385961
AGGTGCT679400.068.2277230
GGTGCTC677900.068.2125831
AGTAGGT679800.068.2099627
GGATCTT776000.068.1957519
GTAGGTG680100.068.1901728
TGAGTAG680600.068.1812225
Produced by FastQC (version 0.11.5)