FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_DJ30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_DJ30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6420395
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG1130471.7607483651706786No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACGGGACCTTATTTTTCAA352750.5494210247188841No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACTTGCTATTATAAAATAA221260.3446205412595331No Hit
AACGAGGTAAACTAATTTTGATCGCAATCGACGTCGTCAAGCTGGCAAAA170800.26602724598720173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133450.207853255134614No Hit
GAGAACAGTGTTCGAAATATATTTCAACTGTACGAACATCAAACGTTTCA130420.20313391933050848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG3156450.069.7630841
GAGAACA2991900.069.447951
AGAACAG3021050.069.0760352
ACGAGGT3200750.068.999932
CGAGGTA3220450.068.7865143
GTACGCC2700.068.70309430
GAACAGT3051750.068.641483
CTGTACC876050.068.5936128
CTGTACT1336250.068.55932628
GAGGTAA3234650.068.509434
ACTGTAC3034600.068.499427
GGTAAAC3237000.068.4969946
AACAGTG3059100.068.478844
ACAGTGT3073750.068.340355
GTGTTCG3076850.068.3357248
GTAAACT3245000.068.322737
TGTACCT279350.068.32049629
GTACTGC41550.068.31467430
AGGTAAA3244050.068.3125
TGTACTC304900.068.28899429
Produced by FastQC (version 0.11.5)