FastQCFastQC Report
Sat 3 Aug 2024
H7G3VDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3VDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868860657
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGACGAGTACTAC563023443.012656068771852No Hit
TCATCTCGAACTAAGC510144802.729710201181682No Hit
CCCACAGGATTCGCAA415067452.2209652091787815No Hit
TACTAACCTCTTGTGA412347572.206411529160892No Hit
AGATCTCGGTGGTCGC379512192.0307142139169128Illumina Single End PCR Primer 1 (100% over 16bp)
TCAAGCAACTAGGTTC375781992.0107544593679143No Hit
TGGTCCTTGTTGGGAG375315082.008256092257177No Hit
GCACTGTCTGCACCCA368184891.9701034885662958No Hit
GGCTTACCTCTCGATA353553621.89181370304806No Hit
CCGTACGGATACCAAC349036841.8676450739794241No Hit
CCTCCCTTGGAGTAAT335335271.7943299771653334No Hit
TACATCCATCGTGTAG332657651.7800024242256816No Hit
CAATTCGGAGCTGGCT324730151.7375835313547403No Hit
CTGAAGTACTCCCAGG314107511.6807433385869603No Hit
TGAAAGAACTCGTCCT286324501.5320805161558921No Hit
AGGAAGCACGCCAGCT284870861.524302301153317No Hit
TTTCGGCTGCTGGAAG262587181.4050655891141701No Hit
CCCGACAGATTCGGGC259271761.3873252616714484No Hit
TTCAGTTGTCGTGTAG258939251.3855460493007745No Hit
GGTTCACCTCGTAACT258775421.3846694189357105No Hit
CAGGGCTTGTGGGCAA254580191.3622213568809696No Hit
CACGGCTAGTGTGTAG251686331.3467367353327508No Hit
AGCTCTGCTATCATCG250103751.3382685812514272No Hit
CGCTCCACGAGTGTAG248584511.3301393502447734No Hit
CGACAGCACACAGATA247215801.322815583248698No Hit
CGTGTACCAGGTGTAG240818051.2885821588570152No Hit
TTGCTGCCGAGTGTAG239617021.282155623012829No Hit
TATCGATCTCTGGCAC237011661.2682147227630358No Hit
CGCAGCAATTGTGTAG234463641.2545806404655884No Hit
AGTCAGACGAGTGTAG234148641.2528951215435642No Hit
CCTTCTAACAGTGTAG232758061.2454543313766169No Hit
ATTCAGGTGTTCGCCT229208781.2264626532827696No Hit
TTGCTCTATTGTGTAG228155151.2208248332770162No Hit
CCAGTCGACGGTGTAG226016371.2093805343562327No Hit
CACATCCTGCAGGGTA224713761.2024104587910966No Hit
AGCTACTATAGTGTAG223131211.1939424652353843No Hit
TCACCAACTTGTGTAG221489621.1851585572760013No Hit
AAGATACACGGTGTAG221233971.1837906115223014No Hit
CACTATCAACGTGTAG218804981.1707934413432302No Hit
TCGCTATGAGGTGTAG215910721.1553066794535234No Hit
TGTCGCTGGTGTGTAG214193311.146117069765036No Hit
ACAGTGTATGGTGTAG213357451.141644505174042No Hit
TAATGTGTCTGTGTAG209201821.1194083369266412No Hit
AACCATAGAAGTGTAG207809441.1119579152229968No Hit
TATGCCTTACGTGTAG205576891.1000118667488221No Hit
TTCATGGTTCGTGTAG204876061.0962618279357357No Hit
CCATCTCGCCGTGTAG204818771.0959552775260761No Hit
TTGACTAGTAGTGTAG203689021.0899101505351023No Hit
AACGGTCTATGTGTAG202660681.0844076536199456No Hit
TCACACAGAGAACAGC199991951.070127669769871No Hit
AATGGATTGAGTGTAG199059091.065136072367968No Hit
CCTGACCACTGTGTAG198584581.062597038768953No Hit
GTTCTTCCTAGGTGAC197848841.0586602016524767No Hit
TATCTTGTAGGTGTAG194451851.0404834050717566No Hit
CCATCATTAGGTGTAG192953811.0324676121639818No Hit
TACACGTTGAGTGTAG190674991.0202739796881497No Hit
AGAGGCAACCGTGTAG190320801.0183787608088108No Hit
TATGATGGCCGTGTAG189718151.015154068814024No Hit
GTTCGCTTGAAGGTGC187384491.0026669955201482No Hit
GGAATTGTAAGTGTAG180939340.9681799406621037No Hit
TGTAATCGACGTGTAG179910260.9626734841151938No Hit
GGGCAATGAAGTCGGA175927250.9413609802370622No Hit
CACATCCTGCCTGCGA165502810.8855813266767272No Hit
AGCCTATGATGTGTAG159253600.8521427180967189No Hit
TCACAACAGTGTGTAG157857110.8446703043842824No Hit
GTGAGATCTTTTCGCC134999910.722364770719233No Hit
TGACTCAGAACAGATA130554150.698576159281842No Hit
TGAGAACCTGCTGGCT120636850.6455101376774287No Hit
AGTACAAGACATGATC113171170.6055623760717865No Hit
GATGAATGATGTTGTG110116510.5892173372452776No Hit
GGCGCAACTGGTTGAG98583500.5275058877757732No Hit
TACTAGCGATGCGGCT66856850.3577412245775582No Hit
TGCCTATGATGTGTAG60717280.3248892835994888No Hit
GGGGGGGGGGGGGGGG41073670.21977919994278097No Hit
TTGGGACGAGTACTAC35154270.18810535642840065No Hit
TGATCTCGGTGGTCGC21711480.11617495354015578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)