FastQCFastQC Report
Sat 3 Aug 2024
H7G3VDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3VDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868860657
Sequences flagged as poor quality0
Sequence length34
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG808748174.327493154563208No Hit
GTAAGCCTGAGGCGGAGACGGTGTTACGGTTATC516148392.7618345330708087No Hit
TGCGGTATGAGGCGGAGACGGTGAGGGACGTATC376687472.015599550394944No Hit
TGCATTCGGAGGCGGAGACGGTGAGGGACGTATC345663561.849595146140422No Hit
TAGACGGTTGAGGCGGAGACGGTGGTTCCTTCAT341219821.8258173434275469No Hit
GGATCACTGAGGCGGAGACGGTGAGGGACGTATC333441701.7841977610854056No Hit
TGCGGTATGAGGCGGAGACGGTGAGTAAACCATC322106441.723544442939172No Hit
TCTGTCCAATGAGGCGGAGACGGTGAGGGACGTA317237841.6974932765145154No Hit
CTCTGCAGCGATCTCGTATGCCGTCTTCTGCTTG312552501.6724227075427165RNA PCR Primer, Index 33 (96% over 28bp)
GTAGGAACTGAGGCGGAGACGGTGAGTAAACCAT310900211.6635815454485219No Hit
TCTGTCCAATGAGGCGGAGACGGTGTCATGAAAA302710831.6197613710052006No Hit
GGATGGAATGAGGCGGAGACGGTGGTTCCTTCAT267334881.4304698373239926No Hit
GAACAGCTATGAGGCGGAGACGGTGTTACGGTTA264733641.4165509825915288No Hit
ACTCGGCAATATCTCGTATGCCGTCTTCTGCTTG259711461.3896780320524453Illumina PCR Primer Index 6 (96% over 31bp)
AAGCGATCGAGGCGGAGACGGTGCCGTTTAGATC246821011.3207031197082983No Hit
CAGCCGCGTAATCTCGTATGCCGTCTTCTGCTTG245832841.3154155665871028Illumina PCR Primer Index 12 (96% over 28bp)
GAACAGCTATGAGGCGGAGACGGTGAGTAAACCA237985401.2734250630650417No Hit
TTGCTCTCATGAGGCGGAGACGGTGAGTAAACCA235113661.2580588024011252No Hit
CCTGCGGAACATCTCGTATGCCGTCTTCTGCTTG234386711.2541689992888538Illumina PCR Primer Index 7 (96% over 28bp)
ATACCAACGCATCTCGTATGCCGTCTTCTGCTTG233433901.2490706523552226Illumina PCR Primer Index 3 (96% over 28bp)
GAGAATGGTTATCTCGTATGCCGTCTTCTGCTTG233433441.2490681909625112Illumina PCR Primer Index 10 (96% over 28bp)
AGTGTTGCACATCTCGTATGCCGTCTTCTGCTTG232109981.241986550097298TruSeq Adapter, Index 18 (96% over 27bp)
TGTCGCTGGTATCTCGTATGCCGTCTTCTGCTTG228532401.2228434428431547RNA PCR Primer, Index 34 (96% over 28bp)
TTCATAAGGTATCTCGTATGCCGTCTTCTGCTTG227587251.2177860834490242Illumina PCR Primer Index 3 (96% over 28bp)
GGTTGCGAGGATCTCGTATGCCGTCTTCTGCTTG226251121.2106366472671697RNA PCR Primer, Index 26 (96% over 28bp)
GAACTGAGCGATCTCGTATGCCGTCTTCTGCTTG224314011.200271455016242Illumina Paired End PCR Primer 2 (96% over 29bp)
CGTCTCATATATCTCGTATGCCGTCTTCTGCTTG221831881.186989940470452Illumina PCR Primer Index 11 (96% over 27bp)
GTCCGTAAGCATCTCGTATGCCGTCTTCTGCTTG219916801.1767426275269917Illumina PCR Primer Index 3 (96% over 29bp)
GGTAACTCGCATCTCGTATGCCGTCTTCTGCTTG217556671.1641139171351285TruSeq Adapter, Index 18 (96% over 28bp)
GACTGAGTAGATCTCGTATGCCGTCTTCTGCTTG212547551.1373108487456378RNA PCR Primer, Index 23 (96% over 30bp)
CCGTATGTTCATCTCGTATGCCGTCTTCTGCTTG212456891.136825740347318Illumina PCR Primer Index 11 (96% over 27bp)
CTTGCACTATGAGGCGGAGACGGTGAGTAAACCA211022361.1291497801593455No Hit
TATGCCTTACATCTCGTATGCCGTCTTCTGCTTG210035031.1238667217552754RNA PCR Primer, Index 39 (96% over 28bp)
CACAGTGTGAGGCGGAGACGGTGGTTCCTTCATC206276881.103757410844783No Hit
CACAGTGTGAGGCGGAGACGGTGGACGCCGAATC205554761.0998934523559827No Hit
ACTTCAAGCGATCTCGTATGCCGTCTTCTGCTTG202913901.0857625967991043RNA PCR Primer, Index 42 (96% over 29bp)
CTTAGTCCATGAGGCGGAGACGGTGTCATGAAAA202860871.085478841026295No Hit
ACCGTTACAAATCTCGTATGCCGTCTTCTGCTTG201722081.0793853423176858Illumina PCR Primer Index 4 (96% over 28bp)
TATGCCAGATGAGGCGGAGACGGTGTCATGAAAA201462961.077998829101575No Hit
CTGTTGGTCCATCTCGTATGCCGTCTTCTGCTTG198173181.0603956975482522TruSeq Adapter, Index 16 (96% over 28bp)
AACCATAGAAATCTCGTATGCCGTCTTCTGCTTG197735431.0580533613320109RNA PCR Primer, Index 17 (96% over 29bp)
TAATGTGTCTATCTCGTATGCCGTCTTCTGCTTG197189111.0551300829273116RNA PCR Primer, Index 16 (96% over 28bp)
TCTATCCTAAATCTCGTATGCCGTCTTCTGCTTG197144061.0548890269671936Illumina PCR Primer Index 11 (96% over 28bp)
CCATCTCGCCATCTCGTATGCCGTCTTCTGCTTG196554251.0517330399341807RNA PCR Primer, Index 41 (96% over 28bp)
GTCTAATGGCATCTCGTATGCCGTCTTCTGCTTG191275191.0234855620913208RNA PCR Primer, Index 34 (96% over 30bp)
GATCGAAGGAGGCGGAGACGGTGTTACGGTTATC190489701.019282520002239No Hit
CTCGCTTCGGATCTCGTATGCCGTCTTCTGCTTG190375351.0186706498792755Illumina PCR Primer Index 9 (96% over 28bp)
CGGTAAGTATGAGGCGGAGACGGTGGACGCCGAA189576021.0143935519746992No Hit
GACACCATGTATCTCGTATGCCGTCTTCTGCTTG189128561.0119992589688296Illumina PCR Primer Index 2 (96% over 30bp)
AGAGGCAACCATCTCGTATGCCGTCTTCTGCTTG188494941.0086088510343123RNA PCR Primer, Index 36 (96% over 29bp)
AGGTCAGATAATCTCGTATGCCGTCTTCTGCTTG186191940.9962858349155135Illumina PCR Primer Index 7 (96% over 30bp)
CCATCATTAGATCTCGTATGCCGTCTTCTGCTTG183506490.9819163847912284RNA PCR Primer, Index 21 (96% over 28bp)
TCAGAAGGCGATCTCGTATGCCGTCTTCTGCTTG183407080.9813844564228525RNA PCR Primer, Index 33 (100% over 29bp)
TCAGCGTTATGAGGCGGAGACGGTGAGTAAACCA181720220.972358315315533No Hit
ATCGGTTGGAGGCGGAGACGGTGTTACGGTTATC172247050.9216687683740992No Hit
CCTGTCTGTCATCTCGTATGCCGTCTTCTGCTTG155752790.8334103958827145Illumina PCR Primer Index 7 (96% over 27bp)
AGGATGTGCTATCTCGTATGCCGTCTTCTGCTTG152324640.8150668667000571TruSeq Adapter, Index 25 (96% over 28bp)
GTCCTTGTTGAGGCGGAGACGGTGCCGTTTAGAT151270390.8094257291649969No Hit
TGATTATACGATCTCGTATGCCGTCTTCTGCTTG143973930.7703834390259734Illumina PCR Primer Index 1 (96% over 28bp)
CCTTGAACGAGGCGGAGACGGTGCCGTTTAGATC139779870.7479416374700856No Hit
CTCCAACACGTGAGGCGGAGACGGTGGTTCCTTC138103130.7389696470024196No Hit
AGCGTAACCGTGAGGCGGAGACGGTGGACGCCGA132863370.7109324577107837No Hit
TAAGCATCCAATCTCGTATGCCGTCTTCTGCTTG129296810.6918483168646425Illumina PCR Primer Index 4 (96% over 28bp)
GTGGTCAACGTGAGGCGGAGACGGTGGACGCCGA128398010.6870389695404668No Hit
ACTCTAGCCGTGAGGCGGAGACGGTGGACGCCGA108358710.5798116065750106No Hit
GCGTAATGTGAGGCGGAGACGGTGCCGTTTAGAT104762800.5605704181721666No Hit
GCTACATCCGTGAGGCGGAGACGGTGGACGCCGA90446580.4839664191186406No Hit
CTCCAACACGTGAGGCGGAGACGGTGAGACTACT88918390.47578929797118635No Hit
CGGTAAGTATGAGGCGGAGACGGTGCCGTTTAGA82713590.44258832080491484No Hit
TTAGACGCCGTGAGGCGGAGACGGTGAGACTACT78302300.41898415329527694No Hit
AGTCAGACGAATCTCGGATGCCGTCTTCTGCTTG70711650.37836769550015736RNA PCR Primer, Index 46 (96% over 27bp)
AGTCAGACGAATCTCGTATGCCGTCTTCTGCTTG67598960.36171214663223555Illumina PCR Primer Index 4 (96% over 29bp)
CCTAGCTTTGAGGCGGAGACGGTGCCGTTTAGAT62070580.3321305939397278No Hit
TCTGTCCAATGTGGCGGAGACGGTGAGGGACGTA45836010.2452617846510747No Hit
TCTGTCCAATGTGGCGGAGACGGTGTCATGAAAA43085130.23054222816784353No Hit
TAAGCATCCAATCTCGGATGCCGTCTTCTGCTTG41766700.22348750209684573RNA PCR Primer, Index 15 (96% over 26bp)
GTATTCGGTGAGGCGGAGACGGTGTTACGGTTAT40117960.21466533553335967No Hit
GAACAGCTATGTGGCGGAGACGGTGTTACGGTTA35960960.1924218366163615No Hit
GAACAGCTATGTGGCGGAGACGGTGAGTAAACCA33399090.17871364499488204No Hit
TTGCTCTCATGTGGCGGAGACGGTGAGTAAACCA32674470.17483630937177996No Hit
CTTGCACTATGTGGCGGAGACGGTGAGTAAACCA29827820.15960430162771627No Hit
TATGCCAGATGTGGCGGAGACGGTGTCATGAAAA28719930.15367614429907708No Hit
GATCGAAGGAGGCGGAGACGGTGAGACTACTATC27847940.14901025336315377No Hit
CTTAGTCCATGTGGCGGAGACGGTGTCATGAAAA27532260.14732109586059952No Hit
TCAGCGTTATGTGGCGGAGACGGTGAGTAAACCA25373760.1357712781044435No Hit
GTCCTTGTTGAGGCGGAGACGGTGCCGTTTATAT22326110.11946374876251675No Hit
GCGTAATGTGAGGCGGAGACGGTGCCGTTTATAT21831030.11681464810246686No Hit
TGATTATACGATCTCGGATGCCGTCTTCTGCTTG19682750.1053195160713365RNA PCR Primer, Index 22 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)