FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764388
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3535081.4274853067235096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC8500.015.8786031
CGCATAT4153.5989935E-511.6151571
CCGATCT10950.011.433862
CGGGTAT4751.4191853E-410.147981
CCGGGAT10951.8189894E-1210.124811
CGCACTT5951.2959708E-59.7215931
GTCGATT5153.2024746E-49.3597871
CGACAGT9103.9875886E-89.0049381
CCCGATC7452.0057998E-58.4027362
CGGGATT7502.1540634E-58.355171
CTACCGT5850.00132050058.1183558
TACCGTA5400.0058423557.91306929
CGGGGAT11251.4543184E-77.7124641
CGGGATC11451.9880099E-77.57008362
TCGGAGT9052.7905844E-57.44925982
CGGGCAT16902.7284841E-117.41583161
CGGTTCG4251.2471103E-47.25625612-13
GAATCCG9901.2735289E-57.24778374
TTTGATC26800.07.18718532
TCGAATC6700.00436301067.18718532
Produced by FastQC (version 0.11.5)