FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm228R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm228R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15773012
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT28900.011.688831
TACCGTA14400.010.22473059
AAAGGGG153550.09.46580180-81
AGGGGGG169300.09.06209582-83
CTACCGT19900.08.5921558
CGGGGTT54700.08.3501541
CGGGATT30800.08.3416821
CGGGGAT50950.08.0309111
GTCCGAT14002.8012437E-107.81646441
AAGGGGG143750.07.68246980-81
GGGGAAT85250.07.59023241
CCGGGAT44600.07.5741531
CGGGTCT57450.07.44298273
GCGTCGT138000.07.38300794-95
GATCGGA315900.07.382728694-95
CGCAAAT23250.07.36699061
CTCGTAT15051.2860255E-97.271131
CGCGGAG51350.07.2271121
ATCGGAA299350.07.148264494-95
AGATCGG354300.07.125530294-95
Produced by FastQC (version 0.11.5)