FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm227R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm227R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16028089
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT16250.011.4193461
TACCGTA15750.010.8561269
CGCGTAT3550.001656527310.722391
CGACCGT12100.09.8307051
CGCGGGT32100.09.6347021
CTACCGT19900.09.0694468
AGGGGGG155150.08.985476582-83
CGGGGAT50250.08.7112761
CCCGGAT53900.08.20964051
AAAGGGG153200.07.999184680-81
CCCGTAT24250.07.455931
ACTACCG24950.07.42420057
AAGGGGG144750.07.41608480-81
GATCGGA291350.07.377053394-95
CGGCGAT28450.07.35868741
CGCCAAC40800.07.3470061
GCGTCGT126600.07.334869494-95
AGCGTCG145350.07.287342594-95
CGCATAT10504.0280593E-67.2503781
ATCGGAA273350.07.23727894-95
Produced by FastQC (version 0.11.5)