FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm225R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm225R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15999807
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA14900.012.75046359
CGCGGGT30150.010.5734851
CGGGGAT50100.010.4468561
AGGGGGG172450.010.17719182-83
AAAGGGG162350.09.83019980-81
AAGGGGG156600.09.82717280-81
TAGGGGG58100.09.44268350-51
CGACCGT11655.456968E-129.3936111
CTACCGT20100.09.2154838
AAAAGGG177950.08.9684278-79
CCGGGAT43600.08.9486611
CGCGAGT15400.08.6510321
CGGGTAT15550.08.2615981
CGGTAAT15453.6379788E-128.0071051
CGACGAT16151.8189894E-127.95466471
CGGGCTT53950.07.93746331
CGCGTAC10859.840733E-87.8935691
CTCGGTT29600.07.87653161
CGACCTA9053.949437E-67.8732124
CGGGTGT47550.07.80503271
Produced by FastQC (version 0.11.5)