FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm220R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm220R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15035353
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT31700.011.7070791
TACCGTA14750.010.62612159
CGGGGAT49700.09.6689261
CCGGGAT44250.09.0319021
CTACCGT19450.08.7908618
GGCGAAT19650.08.232461
GGATCGA19050.07.7299884
TCGCGTT9600.07.66906130-31
GTCCGAT13751.5934347E-97.61260371
CGGGTAT15202.0190782E-107.5124381
CGCCAAT26050.07.48840571
GATCGGA274250.07.481920294-95
CGGGCAT62700.07.36067151
GCGGGTC52650.07.31347662
CCCGGTT33200.07.3087881
AATCGAA27950.07.30754145
AGGGGGG135850.07.29005982-83
AGATCGG306900.07.266322694-95
ATCGGAA257800.07.194723694-95
AGCGTCG141450.07.11883494-95
Produced by FastQC (version 0.11.5)