FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm219R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm219R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432489
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT34400.011.0651171
TACCGTA15850.09.8886429
CGGGGAT52200.09.8441391
CGCGAAT10901.3133103E-98.7302761
CGGGTAT16450.08.6772041
GGGGAAT88050.08.2677351
CCGGGAT46400.08.1009051
AGGGGGG141450.07.605964782-83
CTCGATT31450.07.56438881
GCGTCGT136500.07.55122594-95
GGCGAAT21500.07.52427961
CGGGTCT65850.07.4316993
CGCTAAT7703.0758278E-47.41506531
GTCGTAT13601.0324584E-87.3469121
AGCGTCG159100.07.21003494-95
CGCCAAT29100.07.19422771
GTCGATT14655.7170837E-97.145121
CGACCGT14001.8033461E-87.13700061
GTCGAAT15452.224624E-97.08310751
AGATCGG349900.07.07268294-95
Produced by FastQC (version 0.11.5)