FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm218R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm218R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15169523
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA13500.011.6100479
CGCGGGT31700.010.9569861
CGCGTAT3200.00937610.4081981
CCCGTAT23150.09.865471
CGGGGAT46350.09.3415561
CGACCTA9206.1136234E-99.2940054
CGTATCG18750.08.8687493
CGGGTCT60350.08.6598623
CCGGGAT41350.08.630051
AAGGGGG128650.08.36259180-81
GTATCGA19900.08.3547454
CTACCGT18300.08.30522258
CGGGTAT15757.2759576E-127.8545321
GGGGAAT77300.07.5709981
GCGGGTC52000.07.49711232
GCGGAAT27000.07.4013861
CGCGGAT19350.07.37679671
CCGTATC29050.07.3646122
TCGGATA16853.45608E-117.335962
AGGGGGG143250.07.178698580-81
Produced by FastQC (version 0.11.5)