FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n02_ALm217R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n02_ALm217R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15686950
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG54850.011.25766850-51
CGCGGGT32150.011.2484781
AAAGGGG163800.010.45367480-81
AGGGGGG180250.010.18488182-83
TCGGATT25650.09.0829032
CGGGATT25400.08.9922561
CGCGGTT19250.08.8988291
CGCGATT5355.33472E-48.894211
GGGGGGT175100.08.72106714-15
CGCGAAC8251.0256463E-68.651641
CGACCGT13005.820766E-118.4187121
TACCGTA14053.6379788E-118.11315259
CGGGTCT60600.07.99729063
CGGGGAT48850.07.89008331
CGGGTAT16950.07.8604871
CTACCGT20600.07.83907848
CGGGAAT35700.07.73073771
CGGGGTT48750.07.7110521
CTCGGGT40750.07.706861
AAGGGGG156900.07.659054380-81