FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764388
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3947221.5939097707562973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27000.021.11099444-45
AGAGCAC95500.021.0330758
TCTCGTA28900.020.46082942-43
TAATGCG8500.019.55417334-35
CGTCTGA53550.018.66674216-17
ACGTCTG51750.018.627514-15
CGCGAAA9450.018.59364936-37
CACGTCT55600.018.06360614-15
ATCTCGT31600.018.03623642-43
ACACGTC54000.018.02690112-13
ATGCCGT37250.017.46987748-49
CCGCGAA11300.017.02028834-35
CGGAAGA120600.016.971234
TCGGAAG119250.016.7309883
CACACGT60500.016.71803312-13
GCGAAAT11950.016.6907636-37
ATCGGAA121000.016.4136092
GATCGGA122250.015.7716261
TATCTCG10350.014.68340340-41
AATGCGC11200.014.62821834-35
Produced by FastQC (version 0.11.5)