FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm228R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm228R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15773012
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT537390.3407022070356632TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG73650.037.08370248-49
CGCGAAA78300.037.06693636-37
CGTATGC73650.036.95471646-47
TCGTATG71900.036.6650344-45
CTCGTAT75300.035.32490544-45
GCGAAAT89200.032.35107436-37
CCGCGAA90150.031.90477634-35
CGAAATC91700.031.28778638-39
TCCGCGA94000.031.1286434-35
TCTCGTA85300.031.10012442-43
AATCTCG91750.029.30272340-41
ATCTCGT93750.028.8289642-43
ACTCCGC102500.028.47659932-33
ATGCCGT101800.026.33929648-49
CACTCCG112900.025.62271330-31
GTATGCC107400.025.51875746-47
CTCCGCG113550.025.41260532-33
GCCGTCT107750.024.51012450-51
CCGTCTT111500.024.21829652-53
TGCCGTC120500.022.84306550-51
Produced by FastQC (version 0.11.5)