FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm227R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm227R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16028089
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT476770.29745904206047274TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA73850.036.05263536-37
TATGCCG69500.035.7099348-49
TCGTATG67900.035.53705644-45
CGTATGC70800.035.12132646-47
CTCGTAT70850.034.0909144-45
GCGAAAT82950.032.06886336-37
CGAAATC85350.030.77751438-39
CCGCGAA87450.030.4458234-35
TCTCGTA80650.029.97788242-43
TCCGCGA89950.029.86368234-35
AATCTCG84650.028.87075440-41
ATCTCGT88900.027.62334642-43
ACTCCGC97550.027.51118732-33
ATGCCGT98100.025.39592648-49
CACTCCG108650.024.67959430-31
CTCCGCG111550.024.05841832-33
GTATGCC106250.023.82790246-47
CCGTCTT107150.023.05147452-53
GCCGTCT107900.022.4730450-51
TGCCGTC121450.020.80662350-51
Produced by FastQC (version 0.11.5)