FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm226R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm226R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15356457
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT656740.4276637508248159TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG85850.039.31076444-45
CGCGAAA93450.039.14097236-37
CGTATGC89350.039.0999246-47
TATGCCG90050.038.6377348-49
CTCGTAT88500.038.61670744-45
CCGCGAA103900.034.83851634-35
GCGAAAT105850.034.1742736-37
TCCGCGA109450.033.6361434-35
TCTCGTA100850.033.53449642-43
CGAAATC107750.033.50553538-39
AATCTCG101600.033.38170240-41
ATCTCGT107450.031.8725342-43
ACTCCGC120050.030.70350632-33
GTATGCC120600.028.9486146-47
ATGCCGT119600.028.85307748-49
CACTCCG129700.028.23648330-31
CTCCGCG131000.027.95577232-33
GCCGTCT124400.026.80429850-51
CCGTCTT126900.026.76283552-53
TGCCGTC136400.025.3515650-51
Produced by FastQC (version 0.11.5)