FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm225R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm225R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15999807
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT639940.3999673246058531TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84350.039.13690644-45
TATGCCG89450.037.96753748-49
CGTATGC89200.037.94082646-47
CGCGAAA94800.037.9311536-37
CTCGTAT90950.036.79298844-45
GCGAAAT106750.033.59599736-37
CCGCGAA106450.033.37831534-35
TCCGCGA109150.033.07488334-35
TCTCGTA102150.032.6426642-43
AATCTCG104150.032.2674640-41
CGAAATC110350.032.1771338-39
ATCTCGT111650.030.24806842-43
ACTCCGC124000.029.22749932-33
ATGCCGT119950.028.15503748-49
GTATGCC122500.027.80160146-47
CACTCCG130600.027.46048230-31
CTCCGCG131600.027.23278832-33
CCGTCTT122750.027.08714952-53
GCCGTCT122300.026.79843350-51
TGCCGTC138850.024.27131350-51
Produced by FastQC (version 0.11.5)