FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm220R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm220R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15035353
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT348150.23155425748899944TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52450.033.96039644-45
CGTATGC54650.033.1582346-47
CTCGTAT54050.032.6475144-45
TATGCCG56100.032.17419448-49
TCTCGTA63950.026.96205942-43
ATCTCGT71650.024.39599842-43
CGGCTAT80900.022.42992634-35
GTATGCC84150.021.92925546-47
CCGGCTA82150.021.68274732-33
ATGCCGT85950.021.11078648-49
CCGTCTT89800.020.47017752-53
GCCGTCT89050.020.2158650-51
TATGATC88100.020.0306938-39
TCACCGG90900.019.9621230-31
ACCGGCT93550.019.70055632-33
GTCACCG94800.018.91541128-29
TGCCGTC103300.017.9329350-51
CGTCTTC110650.016.82757852-53
GATCTCG104050.016.79986240-41
GCTATGA117450.015.71270636-37
Produced by FastQC (version 0.11.5)