FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm219R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm219R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432489
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT403420.24550145750896288TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG61250.034.35469448-49
CGTATGC62450.033.5424446-47
TCGTATG61250.033.46286844-45
CTCGTAT63950.031.93863744-45
TCTCGTA76200.025.93145442-43
ATCTCGT83350.024.13438642-43
CGGCTAT90100.023.46098134-35
CCGGCTA91200.022.60400432-33
GTATGCC93500.022.55588246-47
ATGCCGT94950.022.03633948-49
TATGATC96850.021.06563238-39
TCACCGG103500.020.6067930-31
CCGTCTT106000.020.36650552-53
ACCGGCT103900.020.25249932-33
GCCGTCT104450.019.89564750-51
GTCACCG108750.019.30622328-29
TGCCGTC115700.018.59744850-51
GATCTCG117200.017.12374740-41
GCTATGA129700.016.7556836-37
CGTCTTC127900.016.71207852-53
Produced by FastQC (version 0.11.5)