FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm218R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm218R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15169523
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT468450.3088099737875739TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67600.036.67863546-47
TATGCCG68350.036.34565748-49
TCGTATG68100.035.3979744-45
CTCGTAT70200.034.81269544-45
TCTCGTA81200.029.1022442-43
ATCTCGT88100.027.57776642-43
CGGCTAT91000.027.4568634-35
ATCGGAA180200.026.8075392
GATCGGA182200.026.5135361
CCGGCTA92100.026.48322732-33
TCACCGG100950.025.05605530-31
ATGCCGT99250.024.88640448-49
TCGGAAG196700.024.7037053
GTATGCC101550.024.25259446-47
ACCGGCT104550.024.12462632-33
TATGATC100900.024.0802538-39
CCGTCTT105850.023.87317352-53
GCCGTCT105150.023.64812950-51
GTCACCG107250.022.98631928-29
CGGAAGA216700.022.6215864
Produced by FastQC (version 0.11.5)