FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm217R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm217R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15686950
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT709230.45211465581263405TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG95000.038.77441844-45
CGTATGC96350.038.77342246-47
TATGCCG97000.038.53808248-49
CTCGTAT97000.037.65665444-45
TCTCGTA107200.033.01023542-43
CGGCTAT119650.031.26468534-35
ATCTCGT116800.030.78507242-43
CCGGCTA122100.030.20761132-33
ATGCCGT125550.029.56646348-49
GTATGCC129500.028.75632746-47
CCGTCTT131400.028.5935652-53
TATGATC129700.027.7616638-39
ACCGGCT136600.027.62700532-33
GCCGTCT133900.027.5630750-51
TCACCGG138250.027.28083830-31
TGCCGTC146200.025.74773450-51
GTCACCG143700.025.73385628-29
CGTCTTC152900.024.37095852-53
GATCTCG146850.024.08199340-41
GCTATGA156500.024.0699436-37
Produced by FastQC (version 0.11.5)