FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm212R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm212R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14725916
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT393700.26735178986488856TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC57600.036.57298746-47
TCGTATG55950.035.82627544-45
TAATGCG62650.034.99133734-35
TATGCCG60250.034.53077748-49
CTCGTAT60300.033.83258444-45
TCTCGTA68300.029.31339842-43
AATGCGC74800.029.02181234-35
ATCTCGT75050.027.34150142-43
TGCGCAT87400.024.59331736-37
ATGCGCA90800.024.35251236-37
GTATGCC86500.024.29889346-47
GCGCATC89150.024.08391238-39
CTAATGC95900.022.98216232-33
ATGCCGT88950.022.64170848-49
GCCGTCT92050.021.54378350-51
CGCATCT95800.021.34605238-39
CCGTCTT96900.021.20077152-53
GTCACTA104900.020.98806428-29
CATCTCG97500.020.75413340-41
ACTAATG110150.020.33240132-33
Produced by FastQC (version 0.11.5)