FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm211R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm211R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15715424
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT414220.2635754530071858TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58300.035.9299546-47
TCGTATG57400.035.21066744-45
CTCGTAT61800.033.74136444-45
TATGCCG63050.033.56210748-49
TAATGCG66450.033.45488434-35
AATGCGC76900.028.32185634-35
TCTCGTA72450.027.99471542-43
ATCTCGT80350.026.30635642-43
ATGCGCA92050.024.30556936-37
TGCGCAT89550.024.1619236-37
GCGCATC90650.024.10453238-39
GTATGCC90300.023.11839746-47
ATGCCGT90950.022.8226148-49
GCCGTCT93800.021.59746250-51
CTAATGC102050.021.50381732-33
CCGTCTT98000.021.22924652-53
CGCATCT98600.021.12522738-39
GTCACTA112600.019.72210728-29
CATCTCG105550.019.62121440-41
ACTAATG120150.018.81783932-33
Produced by FastQC (version 0.11.5)