FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm210R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm210R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920639
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT514100.32291417448759435TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67750.037.12314644-45
CGTATGC71250.036.56620446-47
TATGCCG73400.036.04518548-49
CTCGTAT74900.034.2135244-45
TAATGCG82150.033.6816134-35
AATGCGC92200.029.80416134-35
TCTCGTA86050.029.36627442-43
ATCTCGT95050.027.13537442-43
TGCGCAT102150.026.22679536-37
ATGCGCA106400.026.11672636-37
GCGCATC105400.025.77863338-39
GTATGCC109250.024.282346-47
ATGCCGT108450.024.2424348-49
CCGTCTT106500.024.19570252-53
GCCGTCT106650.023.64948850-51
CTAATGC119300.023.29182632-33
CGCATCT115500.022.4756738-39
CATCTCG118350.021.61293440-41
GTCACTA129100.021.52461828-29
ACTAATG133800.020.94517532-33
Produced by FastQC (version 0.11.5)