FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm209R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm209R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18015892
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT607290.33708572409292864TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG85400.037.93284248-49
TCGTATG83350.037.6975544-45
CGTATGC85800.037.61759646-47
CTCGTAT89300.035.5581244-45
TAATGCG97950.034.77155334-35
AATGCGC107600.031.5868834-35
TCTCGTA102100.030.379242-43
ATCTCGT108300.029.1444242-43
ATGCGCA126500.027.18675836-37
TGCGCAT124400.026.9965636-37
ATGCCGT124050.026.01846148-49
GCGCATC128550.025.9587438-39
GTATGCC126500.025.77738846-47
GCCGTCT126450.024.84848450-51
CCGTCTT128850.024.8464552-53
CTAATGC137900.024.74867832-33
CGCATCT137750.023.43188538-39
CATCTCG137500.022.95574640-41
ACTAATG152150.022.71174632-33
GTCACTA152150.022.5719228-29
Produced by FastQC (version 0.11.5)