FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm204R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm204R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14811453
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT341400.23049730502469945TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC55450.033.9648746-47
TATGCCG55700.033.72714648-49
TCGTATG54400.033.3980344-45
CTCGTAT60100.030.98131844-45
TATCTCG62650.028.4702440-41
TCTCGTA70100.025.37593842-43
ATCTCGT77050.023.95006642-43
GCTATCT79100.022.7603538-39
GTATGCC83800.021.87920846-47
ATGCCGT87050.021.11691548-49
AGCTATC91650.020.31747638-39
GCCGTCT92500.020.02678150-51
CCGTCTT97350.019.80972152-53
CTATCTC100300.018.63571440-41
TGCCGTC108400.017.94373950-51
GAAGCTA117300.016.58334436-37
AAGCTAT112700.016.52259836-37
CGTCTTC118150.016.04085452-53
AGGGGGG115400.014.44739874-75
AGTCACC142250.013.72418728-29
Produced by FastQC (version 0.11.5)