FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm203R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm203R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15591727
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT384080.24633576511440972TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT58750.034.68446744-45
CGTATGC59950.034.3071846-47
TCGTATG58200.034.2369144-45
TATGCCG60850.033.91690448-49
TATCTCG65400.030.03290240-41
TCTCGTA72550.027.03947642-43
ATCTCGT79800.025.32691442-43
GCTATCT85200.023.10991938-39
ATGCCGT87950.023.0070448-49
GTATGCC89700.022.76996446-47
GCCGTCT94300.021.55852750-51
AGCTATC95800.021.2966438-39
CCGTCTT99300.020.92742752-53
CTATCTC105900.019.28732540-41
TGCCGTC109800.019.2073650-51
AAGCTAT121950.016.88576736-37
CGTCTTC121650.016.7506752-53
GAAGCTA129050.016.63772436-37
AGGGGGG121250.015.41517974-75
CGCGTAT3303.9562874E-614.3939051
Produced by FastQC (version 0.11.5)