FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm202R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm202R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072142
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT368650.2445903176867628TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53100.034.84182444-45
CGTATGC54500.034.46973446-47
TATGCCG54600.034.31960748-49
CTCGTAT58000.031.93923844-45
TATCTCG62550.029.12280340-41
TCTCGTA66950.027.20838242-43
ATCTCGT74550.025.07177242-43
GTATGCC84900.022.2391146-47
GCTATCT82600.021.99650838-39
ATGCCGT85600.021.91852248-49
GCCGTCT90150.021.04936650-51
CCGTCTT93800.020.30623852-53
AGCTATC93150.020.14264938-39
CTATCTC100650.019.06613740-41
TGCCGTC106450.017.98238650-51
CGTCTTC115500.016.63506352-53
AAGCTAT115200.016.49338736-37
GAAGCTA120250.016.49201636-37
AGGGGGG114850.014.619966574-75
GAGCACA294800.012.9227519
Produced by FastQC (version 0.11.5)