FastQCFastQC Report
Sat 10 Aug 2019
H7G3CBGXC_n01_ALm201R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G3CBGXC_n01_ALm201R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17028345
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT407590.2393597263856235TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC62400.036.04321746-47
TCGTATG60450.035.79152344-45
CTCGTAT66250.033.5901444-45
TATGCCG67650.033.28117448-49
TATCTCG72100.029.31741340-41
TCTCGTA78150.027.13817442-43
ATCTCGT85850.025.81068242-43
GTATGCC97950.022.54948646-47
ATGCCGT99550.022.09163548-49
CCGTCTT104750.021.87926352-53
GCTATCT97950.021.7506238-39
AGCTATC106400.020.89382238-39
GCCGTCT107500.020.65674850-51
CTATCTC114250.019.45765740-41
TGCCGTC123200.018.67978350-51
CGTCTTC130550.017.13694652-53
GAAGCTA136550.016.7849236-37
AAGCTAT130550.016.68307536-37
AGGGGGG129250.015.41663374-75
GAGCACA307800.014.6300469
Produced by FastQC (version 0.11.5)