FastQCFastQC Report
Thu 18 Nov 2021
H7FVWAFX3_n01_11617_in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7FVWAFX3_n01_11617_in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234993
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTGG739920.4856713751033558TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTGG572290.37564178729849107TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTGG431780.2834133235243364TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC388080.25472935891732934TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTTGG364860.239488131041478TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTTTGG311540.20448975591915272TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC281940.18506080048740423TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGG249640.16385960925613816TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGATGG237190.15568763306947367TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATGG230720.15144083098692596TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTTTG227100.14906472224831346TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTGG222060.14575654875588062TruSeq Adapter, Index 12 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTTG216840.1423302262101466TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATGC199540.13097478942064494TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTTG165290.10849365011194952TruSeq Adapter, Index 12 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)