FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n02_musca_female_3.352000000b0774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n02_musca_female_3.352000000b0774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548003
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22560.14573615167412468No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG21270.13740283449063084Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA600.00395153731.6663991
CGCCGTA5750.020.65166546-47
GGCGGAT3001.6734703E-1020.5831581
CCGTATC6500.018.27143748-49
GATCTCG6350.017.95231234-35
CGCGGGG1600.001191335817.812351
GGGGGAC2453.7637055E-617.4488321
CTCGGTG6700.017.0142336-37
GCGGAAC2901.0299063E-616.3791711
GCCGGGT2355.1365947E-516.1700761
TCTCGGT7550.016.04239336-37
GGGGTCT1800.002619308215.8331991
GCGGGGT2709.482159E-615.8331991
GCCGTAT7200.015.83294346-47
CGTACTG7550.015.7263118
AGATCTC7100.015.38719332-33
TCGCCGT7600.015.3113844-45
CGTATCA7300.014.96756148-49
GCGGAAT3202.8710583E-614.8436251
GCCGGAT3951.10485416E-714.4302561
Produced by FastQC (version 0.11.2)